The report summarizes the key ACEWATER2 project scientific achievements of the activities implemented by the African CoEs (Centers of Excellence), supporting Institutions, leading experts and the JRC. The outcomes of few ongoing research projects at JRC complements the overall framework. The first three chapters focus on the analysis of the WEFE (Water-Energy-Food-Ecosystem) nexus assessment at regional scale over the two formerly existing networks of CoE, in Western and Southern Africa, and the 2018 newly established network of CoE in CentralEastern Africa.